sna conjugated with biotin  (Vector Laboratories)

Journal: PLoS ONE

Article Title: N -Glycans from Porcine Trachea and Lung: Predominant NeuAcα2-6Gal Could Be a Selective Pressure for Influenza Variants in Favor of Human-Type Receptor

doi: 10.1371/journal.pone.0016302

Figure Lengend Snippet: Sections containing Siaα2-3Gal and Siaα2-6Gal receptors were reacted with DIG-labeled MAA and biotinylated SNA, respectively. DIG-MAA- and biotin-SNA-exposed sections were then reacted with anti-DIG-rhodamine (red) and avidin-fluorescein (green), respectively. Sections from representative tissues are shown at original magnification of x400. E = epithelium, CC = ciliated cells, GC = goblet cells, BM = basement membrane, LP = lamina propria, TBC = terminal bronchiole.

Article Snippet: Immunostaining was begun by adding 50 µl of a lectin mixture of biotin-conjugated Sambucus niga agglutinin (SNA; specific for NeuAcα2-6Gal; 5 µg/mL; Vector Lab, Burlingame, CA) and digoxigenin (DIG)-conjugated Maackia amurensis agglutinin (MAA; specific for NeuAcα2-3Gal; 10 µg/mL; Roche, Mannheim, Germany) to PBS containing 0.05% Tween-20 and 0.5% bovine serum albumin (BSA).

Techniques: Labeling, Avidin-Biotin Assay

Journal: PLoS ONE

Article Title: N -Glycans from Porcine Trachea and Lung: Predominant NeuAcα2-6Gal Could Be a Selective Pressure for Influenza Variants in Favor of Human-Type Receptor

doi: 10.1371/journal.pone.0016302

Figure Lengend Snippet: a, mol % was calculated from the peak area in by comparison with total N -glycan content in each porcine tissue. b, Structures of PA-oligosaccharides are represented by symbols as follows: red diamond, NeuAcα2-6; purple diamond, NeuAcα2-3; green diamond, NeuGcα2-6; light blue diamond, NeuGcα2-3; yellow circle with α, α-galactose(Gal); yellow circle, galactose; blue square, N-acetylglucosamine (GlcNAc); green circle, mannose (Man), red triangle, fucose (Fuc). c, Units of glucose (GU) were calculated from the elution times of the peaks obtained from the ODS column in .

Article Snippet: Immunostaining was begun by adding 50 µl of a lectin mixture of biotin-conjugated Sambucus niga agglutinin (SNA; specific for NeuAcα2-6Gal; 5 µg/mL; Vector Lab, Burlingame, CA) and digoxigenin (DIG)-conjugated Maackia amurensis agglutinin (MAA; specific for NeuAcα2-3Gal; 10 µg/mL; Roche, Mannheim, Germany) to PBS containing 0.05% Tween-20 and 0.5% bovine serum albumin (BSA).

Techniques:

Journal: PLoS ONE

Article Title: N -Glycans from Porcine Trachea and Lung: Predominant NeuAcα2-6Gal Could Be a Selective Pressure for Influenza Variants in Favor of Human-Type Receptor

doi: 10.1371/journal.pone.0016302

Figure Lengend Snippet: Amounts of α2,3- and α2,6-sialyl receptors in comparison with those of the other outer residues present on N -glycans derived from the porcine trachea (upper and lower) and lungs of a pig.

Article Snippet: Immunostaining was begun by adding 50 µl of a lectin mixture of biotin-conjugated Sambucus niga agglutinin (SNA; specific for NeuAcα2-6Gal; 5 µg/mL; Vector Lab, Burlingame, CA) and digoxigenin (DIG)-conjugated Maackia amurensis agglutinin (MAA; specific for NeuAcα2-3Gal; 10 µg/mL; Roche, Mannheim, Germany) to PBS containing 0.05% Tween-20 and 0.5% bovine serum albumin (BSA).

Techniques: Derivative Assay

Journal: PLoS ONE

Article Title: N -Glycans from Porcine Trachea and Lung: Predominant NeuAcα2-6Gal Could Be a Selective Pressure for Influenza Variants in Favor of Human-Type Receptor

doi: 10.1371/journal.pone.0016302

Figure Lengend Snippet: A predominant quantity of Siaα2-6Gal receptor on N -glycans in the trachea (upper and lower) and lungs of a pig.

Article Snippet: Immunostaining was begun by adding 50 µl of a lectin mixture of biotin-conjugated Sambucus niga agglutinin (SNA; specific for NeuAcα2-6Gal; 5 µg/mL; Vector Lab, Burlingame, CA) and digoxigenin (DIG)-conjugated Maackia amurensis agglutinin (MAA; specific for NeuAcα2-3Gal; 10 µg/mL; Roche, Mannheim, Germany) to PBS containing 0.05% Tween-20 and 0.5% bovine serum albumin (BSA).

Techniques: